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Although most anabolic and androgenic effects are expressed by the androgen receptor, some anabolic steroids can function outside the androgen receptorby interacting with the cytochrome P450 2D6 (CYP2D6) enzyme system (Hazell et al., 2006; Li et al., 2007). In this study, we have investigated 3 types of anabolic steroids, and how these steroids interact with CYP2D6 (CYP2D6). Methods Subjects, screening, assays, and study design A total of 42 males (14% of the general population in China) (mean ±SD age: 17 ± 2) who reported not using steroids at least 3 times in the past year voluntarily participated in all 6-week periods of the study. The subject selection process consisted of 3 phases. Phase I was initiated when a subject underwent an evaluation by a research physician, using a simple visual analogue scale, which was included at pre-testing and weekly for the 6-week period. During the pre-testing phase, the subject was asked to report the presence of any of the aforementioned types of anabolic and androgenic steroids in his blood. The pre-testing phase was completed by the first visit to the laboratory. The subject underwent the following assays from the baseline day (day 1). The first assays involved a urine dipstick test on urine collected on the first day of each week, and a fasting urine sample collected on the first day of each week as part of a routine screening examination: the urinary metabolite of androgenic steroids (carnitine, 17-β,17-tetrahydroisoheptanoic acid [C(H))17-β-DH) assay, and a fasting plasma concentration assay (C(A)) for cortisol (CORT). Subjects underwent a total of two additional assays after screening, each measuring plasma concentrations of sex hormone-binding globulin (SHBG) (Bundeskriminalamt, Germany). The third phase involved the administration of the test assays, and a urine dipstick test between days 5-10 of each week for the 6-month period. Subjects underwent a second urine dipstick test after a minimum of 6 weeks to assess anabolism. As such, a urine dipstick test was administered on the third day of the sixth week in each month. During both the baseline, pre-testing, and 6-month period of the study, the subject was required to maintain his or her current androgen status during the testing period. During the 6-month period, subject Related Article:
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